Fm4-64. Common dyes include calcofluor white DAPI DASPMI FM4-64 and DIOC6. Scale bars 10 μm.
Endocytosis is a process by which cells internalize non-particulate materials such as proteins or polysaccharides by engulfing them. For this reason it is important to view them under high magnification using fluorescence microscopy. This lipophilic dye is an important tool for visualizing vacuolar organelle morphology and dynamics for studying the endocytic pathway and for screening and characterizing yeast endocytosis mutants.
All fluorescent signals.
FM4-64 for the vacuoles. DIOC6 for the endoplasmic reticulum and mitochondria. Statistical significance compared to control was assessed by paired t tests p 005 p 001 n 7. 19000 - AT UVDAPI Longpass.